effect of ph on rituximab production

The pH of the culture medium is an important parameter that affects the growth and productivity of cells in a bioreactor. The pH can affect the solubility, stability and activity of enzymes, as well as the uptake and metabolism of nutrients.

In the case of rituximab production, the pH can affect the stability and activity of the protein, as well as the productivity of the cells. Optimal pH for rituximab production in Chinese hamster ovary (CHO) cells usually ranges from 7.0 to 7.5. A pH that is too low or too high can cause the protein to denature or aggregate, reducing its activity and stability. Additionally, a pH that is too low or too high can also cause stress to the cells, reducing their productivity.

A research article "Development of a high-titer, high-density fed-batch process for the production of Rituximab in Chinese Hamster Ovary (CHO) cells" by Wang et al. (2015) aimed to improve the production of rituximab by developing a high-titer and high-density fed-batch process. The study used a CHO cell line that had been modified to express the rituximab gene. The cells were grown in a culture medium containing glucose, amino acids, and a proprietary feed supplement, and the culture conditions were optimized to achieve high yields of the protein.

The results of the study showed that the optimal pH for rituximab production was 7.2 and the productivity of the cells was highest at that pH. Also, the study found that the quality of the protein was similar to that of the clinically used product, with a high degree of purity, stability, and bioactivity.

In conclusion, the pH of the culture medium is an important parameter that can affect the stability and activity of rituximab, as well as the productivity of the cells. It is important to maintain the pH within the optimal range to ensure the stability and activity of the protein and the productivity of the cells.

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