**Subject: Request for Permission to Keep a Small Beard** Dear Sir, I hope this letter finds you in good health. I am writing to formally request permission to maintain a small beard due to my personal and religious beliefs. I understand the importance of maintaining a professional appearance at the workplace and assure you that my beard will be kept neat and well-groomed, in accordance with company standards. I kindly ask for your consideration and approval of this request, and I am more than willing to discuss any concerns you may have. Thank you for your understanding and support. Sincerely, Mohd Aasif Executive Cadila Pharmaceutical Pvt Ltd 23 October 2024 **Subject: Request for Approval to Maintain a Small Beard** Dear [Manager's Name], I hope you are doing well. I am writing to request permission to maintain a small beard, in line with my personal and religious practices. I fully respect the company’s appearance policies and will ens...
The pH of the culture medium is an important parameter that affects the growth and productivity of cells in a bioreactor. The pH can affect the solubility, stability and activity of enzymes, as well as the uptake and metabolism of nutrients. In the case of rituximab production, the pH can affect the stability and activity of the protein, as well as the productivity of the cells. Optimal pH for rituximab production in Chinese hamster ovary (CHO) cells usually ranges from 7.0 to 7.5. A pH that is too low or too high can cause the protein to denature or aggregate, reducing its activity and stability. Additionally, a pH that is too low or too high can also cause stress to the cells, reducing their productivity. A research article "Development of a high-titer, high-density fed-batch process for the production of Rituximab in Chinese Hamster Ovary (CHO) cells" by Wang et al. (2015) aimed to improve the production of rituximab by developing a high-titer and high-density fed-batc...
Removing bovine endogenous retrovirus (BERV) or Bovine exogenous retrovirus (BERV) from plasma protein through DEAE (Diethylaminoethyl) resin anion-exchange chromatography is a common method. DEAE resin is a type of anion-exchange resin that can be used for the purification of plasma proteins. Here are the general steps for removing BET from plasma protein through DEAE resin anion-exchange chromatography: 1. Prepare the sample: The plasma protein sample should be clarified and filtered to remove any debris or contaminants before being loaded onto the column. 2. Equilibrate the column: The column should be equilibrated with a buffer that is compatible with the DEAE resin. A common buffer used is a low salt buffer such as Tris-HCl, pH8.0 3. Load the sample: The plasma protein sample is then loaded onto the column, and the BET proteins are retained by the DEAE resin while the other proteins pass through. 4. Wash th...
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