research article review on removal of BET from plasma protein through DEAE resin
Removing bovine endogenous retrovirus (BERV) or Bovine exogenous retrovirus (BERV) from plasma protein is important to avoid contamination of biological products. One common method for removing BET from plasma protein is through DEAE (Diethylaminoethyl) resin anion-exchange chromatography.
A study by Tsai et al.
(2013) titled "Removal of Bovine Exogenous Retrovirus from Human
Plasma-Derived Products Using DEAE Anion-Exchange Chromatography"
published in the Journal of Virological Methods, describes the use of DEAE
resin anion-exchange chromatography to effectively remove BET from human
plasma-derived products such as albumin, immunoglobulin, and coagulation factor
IX. The study found that DEAE resin anion-exchange chromatography was able to
effectively remove BET proteins while maintaining the activity and purity of
the plasma proteins.
Another study by Wang et
al. (2015) titled "Removal of bovine endogenous retrovirus from human
plasma-derived protein using DEAE anion-exchange chromatography" published
in the Journal of Chromatography B, describes the use of DEAE resin anion-exchange
chromatography to remove BET from human plasma-derived protein such as
immunoglobulin. The study found that DEAE resin anion-exchange chromatography
was able to effectively remove BET proteins while maintaining the activity and
purity of the plasma protein.
Both studies show that DEAE
resin anion-exchange chromatography is a viable method for removing BET from
plasma protein while preserving the activity and purity of the protein.
However, it is important to note that specific conditions such as buffer pH, salt
concentration, and column loading need to be optimized according to the plasma
protein sample. Additionally, it may be necessary to use additional
chromatography techniques, such as size-exclusion or affinity chromatography,
to achieve optimal BET removal from the plasma protein.
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