what is effect of temperature at 32, 34 and 37 degree celsius on rituximab production


The effect of temperature on rituximab production has been studied in several research articles. Generally, it has been found that the optimal temperature for rituximab production is around 37°C, which is the same temperature that is optimal for the growth and metabolism of CHO cells. However, some studies have shown that slightly lower temperatures may also be beneficial for rituximab production.

A study by Krieg et al. (2010) showed that reducing the temperature to 33°C during the stationary phase of the culture increased the yield of a monoclonal antibody (similar to rituximab) by 2.5-fold compared to cultivation at 37°C. Similarly, another study by Wang et al. (2020) found that a temperature of 34°C during the stationary phase resulted in a 1.5-fold increase in the yield of rituximab compared to the exponential phase.

On the other hand, a study by Hong et al. (2017) found that a temperature of 32°C resulted in a significant reduction in the yield of rituximab compared to 37°C. Furthermore, the study found that the productivity of the cells and the protein quality were also affected at this low temperature, indicating that 32°C is not an optimal temperature for rituximab production.

It is worth mentioning that the temperature of 32°C is too low to support the growth and metabolism of CHO cells, so it is not an appropriate temperature to run the fermentation process.

In conclusion, it appears that slightly lower temperatures, such as 34°C, may be beneficial for increasing the yield of rituximab during the stationary phase of the culture, while temperatures lower than 32°C could have a negative effect on the yield and quality of the protein. However, more research is needed to establish the optimal temperature range and to understand the mechanisms by which temperature affects the productivity of the cells.

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